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教育部分子表观学重点实验室

发布时间:2016-01-22文章来源: 浏览次数:

实验室简介

教育部分子表观学重点实验室于2003年12月正式成立,为系(院)级学校直管实验室。实验室主要任务是开展表观遗传学 (Epigenetics) 现象和规律的基础理论研究。项目组研究所覆盖研究方向主要包括三方面:(1)表观遗修饰的生物学意义和作用机制;(2)高等植物远缘杂交与多倍化诱导表观遗传变异的分子机制;(3)表观遗传学变异与植物的环境适应性变异与进化的关系。通过综合应用基因组学、表观遗传学、系统生物学、分子生物学、离子组学、农学等学科的先进技术和方法,基于已有的表观遗传调控基因突变体材料和已成功创制的小麦、水稻等作物独特多倍体体系开展整合性研究。

实验室地址及电话

分子表观遗传学教育部重点实验室位于东北师范大学综合实验楼五楼(509-524)

联系电话:0431-85099367。

研究方向

1、表观遗传学调控机制方面

主要研究水稻中与表观遗传遗传修饰有关的突变体进行研究,以揭示表观遗传修饰在调控植物生长发育、基因表达和基因组稳定性过程中的重要作用。通过对表观遗传调控体系的研究将有助于深入理解水稻的表观遗传调控机制和最终实现可控的分子水平水稻育种策略提供条件。

2、高等植物远缘杂交与多倍化诱导表观遗传变异的分子机制方面

主要在水稻和小麦中开展新合成多倍体核型稳定性研究;植物杂交/多倍化对基因组影响的研究;植物杂交/多倍化对表观遗传影响的研究;植物杂交/多倍化的核质兼容性研究;新合成多倍体群体的功能基因组开发及DNA甲基化和组蛋白修饰对杂种优势形成的作用机制研究。

3、表观遗传学变异与植物的环境适应性变异与进化的关系

在小麦上主要开展在特定环境条件下小麦的倍性、世代、核型稳定性、生理状态、基因组稳定性及特定表观遗传变异与其环境适应的关系;

在水稻上主要开展在特定环境条件下水稻的突变体后代及杂交/多倍化材料表现出某种特定环境抗性的遗传以表观遗传机制研究。

研究队伍

实验室固定的研究人员

教 授:李 玉 蒋继明 刘 宝 宫 磊

副教授:庞劲松 欧秀芳 徐春明

工程师:姜丽丽

人才培养

本实验室设立迄今已经培养了大量硕士、博士与博士后人员,他们中的很多人在国内外的高校和研究所取得了突出成绩,并且成为国内外高校及科研机构的学术带头人。例如,复旦大学王永明教授(中组部青年千人)、华南农业大学刘振兰教授、本实验室引进的宫磊教授(中组部青年千人)等。近些年本实验室的部分博士与硕士毕业生分别在中科中科院植物研究所、中科院应用化学所、吉林省农科院、吉林大学、吉林农业大学、南京农业大学等获得工作岗位。

目前在读的硕士生有20人,博士生17人,博士后研究人员3人。

已毕业硕士生有65人,博士生33人,出站的博士后研究人员2人。

科研成果

2010年至今实验室发表SCI论文

1. Lanjuan Hu, Ning Li, Chunming Xu, Silin Zhong, Xiuyun Lin, Jingjing Yang, Tianqi Zhou, Anzhi Yuliang, Ying Wu, Yun-Ru Chen, Xiaofeng Cao, Assaf Zemach, Sachin Rustgi, Diter von Wettstein, Bao Liu, 2014, Mutation of a major CG methylase in rice causes genome-wide hypomethylation, dysregulated genome expression, and seedling lethality. Proc Natl Acad Sci U S A 111: 10642-10647 IF:9.809;

2. Chunwu. Yang, L. Zhao, H. Zhang, Z. Yang, H. Wang, S. Wen, C. Zhang, S. Rustgi, D. von Wettstein, B. Liu, 2014, Evolution of physiological responses to salt stress in hexaploid wheat. Proc Natl Acad Sci U S A 111: 11882-11887 IF:9.809;

3. Huakun Zhang, Bo Zhu, Bao Qi, Xiaowan Gou, Yuzhu Dong, Chunming Xu, Bangjiao Zhang, Wei Huang, Chang Liu, Xutong Wang, Chunwu Yang, Hao Zhou, Khalil Kashkush, Moshe Feldman, Jonathan F. Wendel, Bao Liu, 2014, Evolution of the BBAA Component of Bread Wheat during Its History at the Allohexaploid Level. The Plant Cell 26: 2761-2776 IF:9.575;

4. Chunming Xu, Yan Bai, Xiuyun Lin, Na Zhao, Lanjuan Hu, Zhiyun Gong, J. F. Wendel, Bao Liu, 2014, Genome-wide disruption of gene expression in allopolyploids but not hybrids of rice subspecies. Molecular Biology and Evolution 31: 1066-1076 IF:14.308;

5. N. Wang, D. Zhang, Z. Wang, H. Xun, J. Ma, H. Wang, W. Huang, Y. Liu, X. Lin, N. Li, X. Ou, C. Zhang, M. B. Wang, B. Liu, 2014, Mutation of the RDR1 gene caused genome-wide changes in gene expression, regional variation in small RNA clusters and localized alteration in DNA methylation in rice. Bmc Plant Biol 14: 177 IF:3.942;

6. D. Zhang, Z. H. Wang, N. N. Wang, Y. Gao, Y. Liu, Y. Wu, Y. Bai, Z. B. Zhang, X. Y. Lin, Y. Z. Dong, X. F. Ou, C. M. Xu, B. Liu, 2014, Tissue Culture-Induced Heritable Genomic Variation in Rice, and Their Phenotypic Implications. Plos One 9 IF:3.534;

7. Huakun Zhang,Yao Bian, Xiaowan Gou, Yuzhu Dong, Sachin Rustgi***,Bangjiao Zhang, Chunming Xu, Ning Li, Bao Qi, FangPu Han, Diter von Wettstein**, Bao Liu, 2013, Intrinsic karyotype stability and gene copy number variations may have laid the foundation for tetraploid wheat formation. Proc Natl Acad Sci U S A 110: 19466-19471 IF: 9.809;

8. ZhenHui Wang, Di Zhang, Yan Bai, YunHong Zhang, Ying Liu, Ying Wu, XiuYun Lin, JiaWei Wen, ChunMing Xu, LingFeng Li, Bao liu, 2013, Genomewide Variation in an Introgression Line of Rice-Zizania Revealed by Whole-Genome re-Sequencing. Plos One 8 IF:3.534;

9. LiLi Jiang, XiaoMing Yu, Xin Qi, Qian Yu, Sen Deng, Bing Bai, Ning Li, Ai Zhang, ChangPu Zhu, Bao Liu, JinSong Pang, 2013, Multigene engineering of starch biosynthesis in maize endosperm increases the total starch content and the proportion of amylose. TRANSGENIC RES 22 IF:2.281

10.YunHong Zhang, XiuYun Lin, XiuFang Ou, LanJuan Hu, JinMing Wang, ChunWu Yang, ShuCai Wang, Bao Liu, 2013, Transcriptome alteration in a rice introgression line with enhanced alkali tolerance. PLANT PHYSIOL BIOCH 68: 111-117 IF:2.352

11.XiaoRan Wang, Rui Wu, XiuYun Lin, Yan Bai, CongDi Song, XiaoMing Yu, ChunMing Xu, Na Zhao, YuZhu Dong, Bao Liu, 2013, Tissue culture-induced genetic and epigenetic alterations in rice pure-lines, F1 hybrids and polyploids. Bmc Plant Biol 13: 77 IF:3.942;

12.ZhenYing Dong, HongYan Wang, YuZhu Dong, YongMing Wang, Wei Liu, GaoJian Miao, XiuYun Lin, DaQing Wang, Bao Liu, 2013, Extensive Microsatellite Variation in Rice Induced by Introgression from Wild Rice (Zizania latifolia Griseb.) Plos One 8: 62317-1-12 IF:3.534;

13.Rui Wu, XiaoRan Wang, Yan Lin, YiQiao Ma, Gang Liu, XiaoMing Yu, SiLing Zhong, Bao Liu, 2013, Inter-Species Grafting Caused Extensive and Heritable Alterations of DNA Methylation in Solanaceae Plants. Plos One 8: 61995-1-11 IF:3.534;

14.ChunYu Zhang, NingNing Wang, YunHong Zhang, QiZhi Feng, ChunWu Yang, Bao Liu, 2013, DNA methylation involved in proline accumulation in response to osmotic stress in rice (Oryza sativa). GENETICS AND MOLECULAR RESEARCH 12: 1269-1277 IF: 0.85;

15.Huakun Zhang, Yao Bian, Xiaowan Gou, Bo Zhu, Chunming Xu, Bao Qi,Ning Li, Sachin Rustgi, Hao Zhou, Fangpu Han, Jiming Jiang, Diter von Wettstein, and Bao Liu, 2013, Persistent whole-chromosome aneuploidy is generally associated with nascent allohexaploid wheat. Proc Natl Acad Sci U S A 110: 3447-3452 IF: 9.809;

16.JinSong Pang, MingYue Dong, Ning Li, YanLi Zhao, Bao Liu, 2012, Functional characterization of a rice de novo DNA methyltransferase,OsDRM2,expressed in Escherichia coli and yeast. BIOCHEM BIOPH RES CO 432:157-162 IF: 2.281;

17.Chunwu Yang, Tianyuan Zhang, Huan Wang, Na Zhao , Bao Liu, 2012, Heritable alteration in salt-tolerance in rice induced by introgression from wild rice (Zizania latifolia). RICE 5 IF: 2.381;

18.Xiufang Ou , Yunhong Zhang , Chunming Xu, Xiuyun Lin, Qi Zang, Tingting Zhuang, Lili Jiang, Diter von Wettstein , Bao Liu, 2012, Transgenerational Inheritance of Modified DNA Methylation Patterns and Enhanced Tolerance Induced by Heavy Metal Stress in Rice (Oryza sativa L.) Plos One 7: 41143-1-11 IF:3.73;

19.XiaoHui Shan, YiDan li, XiaoMing Liu, Ying Wu, MingZhe Zhang, WanLi Guo, Bao Liu, YaPing Yuan, 2012, Comparative analyses of genetic/epigenetic diversities and structures in a wild barley species (Hordeum brevisubulatum) using MSAP,SSAP and AFLP. GENETICS AND MOLECULAR RESEARCH 11: 2749-2759 IF: 0.994;

20.QiZhi Feng, ChunWu Yang, XiuYun lin, JinMing Wang, XiuFang Ou, ChunYu Zhang, Yu Chen, Bao Liu, 2012, Salt and alkaline stress induced transgenerational alteration in DNA methylation of rice (Oryza sativa). AUSTRALIAN JOURNAL OF CROP SCIENCE 6: 877-883;

21.Agboola R S, Kimatu J N, Liao Y C, Bao liu, 2012, Morphological and cytosine DNA methylation changes induced by a combined effect of boron (B) and salt toxicity in Sorghum bicolor inbred line[J]. African Journal of Biotechnology, 11(48): 10874-10881;

22.Wang, H., Ahan, J., Wu, Z., Shi, D., Liu, B., & Yang, C, 2012, Alteration of nitrogen metabolism in rice variety'Nipponbare'induced by alkali stress. Plant and soil, 355(1-2): 131-147 IF:2.638;

23.Yang X, Yu Y, Jiang L, et al,Bao Liu, 2012, Changes in DNA methylation and transgenerational mobilization of a transposable element (mPing) by the Topoisomerase II inhibitor, Etoposide, in rice[J]. BMC plant biology, 12(1): 48 IF:4.354;

24.Wu, Y., Wu, R., Zhang, B., Jiang, T., Li, N., Qian, K., Bao Liu, Zhang, J. , 2012, Epigenetic instability in genetically stable micropropagated plants of Gardenia jasminoides Ellis. Plant Growth Regulation, 66(2):137-14 IF:1.67;

25.Qi, B., Huang, W., Zhu, B., Zhong, X., Guo, J., Zhao, N., ... & Liu, B., 2012, Global transgenerational gene expression dynamics in two newly synthesized allohexaploid wheat (Triticum aestivum) lines. BMC biology, 10(1) IF:6.531;

26.Zhang, M., Xu, C., von Wettstein, D., & Liu, B., 2011, Tissue-specific differences in cytosine methylation and their association with differential gene expression in sorghum. Plant physiology, 156(4):1955-1966 IF:6.535;

27.Liu B, Xu C, Kou H, et al. 2011, Induced Pib Expression and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice. J INTEGR PLANT BIOL, 53(10): 814-823 IF:2.534;

28.Zhao, N., Xu, L., Zhu, B., Li, M., Zhang, H., Qi, B., ... & Liu, B. 2011, Chromosomal and genome-wide molecular changes associated with initial stages of allohexaploidization in wheat can be transit and incidental. Genome, 54(8): 692-699 IF:1.653;

29.Zhao, N., Zhu, B., Li, M., Wang, L., Xu, L., Zhang, H., ... & Liu, B. (2011). Extensive and heritable epigenetic remodeling and genetic stability accompany allohexaploidization of wheat. Genetics, 188(3): 499-510 IF:4.007;

30.高立宏, 路云侠, 柴娜, 刘宝, & 刘立侠. (2011). 水稻转座子 Pong RNAi 载体的构建和农杆菌介导的遗传转化.东北师大学报: 自然科学版, 43(2), 117-123.

31.Ngezahayo, F., Wang, X., Yu, X., Jiang, L., Chu, Y., Shen, B., ... & Liu, B. (2011). Habitat-induced reciprocal transformation in the root phenotype of Oriental ginseng is associated with alteration in DNA methylation. Chinese Science Bulletin, 56(16): 1685-1689 IF:1.321;

32.Yu, X., Li, X., Zhao, X., Jiang, L., Miao, G., Pang, J., ... & Liu, B. (2011). Tissue culture‐induced genomic alteration in maize (Zea mays) inbred lines and F1 hybrids. Annals of Applied Biology, 158(3): 237-247 IF:2.179;

33.Kou, H. P., Li, Y., Song, X. X., Ou, X. F., Xing, S. C., Ma, J., ... & Liu, B. (2011). Heritable alteration in DNA methylation induced by nitrogen-deficiency stress accompanies enhanced tolerance by progenies to the stress in rice (Oryza sativa L.). Journal of plant physiology, 168(14): 1685-1693 IF: 2.791;

34.Wang, X., Ou, X., Wang, K., Dong, M., Man, G., Cai, Y., ... & Liu, B. (2011). Changes in cytosine methylation and DNA sequence induced by ethylmethane sulfonate in rice. Botany, 89(6): 417-424 IF: 1.251.

学术交流与开放

与本课题组有合作的“海外学术大师”及主要“海外学术骨干”成员有Diter von Wettstein(美国科学院、欧共体科学院等7国外籍院士;美国Washington State University杰出教授),James Birchler(美国科学院院士; 国际著名植物遗传学家。University of Missouri-Columbia 杰出教授),Moshe Feldamn(以色列科学与人文科学院院士;国际著名小麦遗传学家; The Weizmann Institute of Science 杰出教授),Jonathan F. Wendel(美国Iowa State University杰出教授、系主任;国际著名进化植物学家),Jiming Jiang(美国University of Weisconsin- Madison终身教授;国际著名植物染色体和表观基因组学专家)Ming-Bo Wang(澳大利亚CSIRO-Plant Industry 资深研究员和澳大利亚国家最高科技奖-总理奖获得者)等。

实验室风采

Diter教授到访进行学术交流

罗明成教授到访进行学术交流

集体合影

实验室一角

组培室培育的幼苗

温室种植冬小麦

温室种植冬玉米

实验室培育的幼苗

实验室培育的幼苗

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